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Ketogenesis was observed in perfused livers and isolated hepatocytes under a variety of conditions including diabetes and a high supply of acetate or ethanol as hepatic fuels (Cohen et al., 1979b, 1981a; Canioni et al., 1985; Cohen, 1987a,b).Liver metabolism gave rise to the synthesis of 3-hydroxybutyrate and acetoacetate in these model systems. In contrast, Seelig and coworkers (Cross et al
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Ketogenesis. Ketogenesis takes place in the mitochondria where fatty acid catabolism generates acetyl-CoA. Acetyl-CoA C-acetyltransferase (thiolase; EC2.3.1.9) joins two acetyl-CoA molecules, and hydroxymethylglutaryl-CoA synthase (HMG-CoA synthase; EC4.1.3.5) adds another one.
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